The 172 addresses are for apps. and can be found Apps -->Configuration → Settings
It may have been all fine until the reboot which since the network was reconfigured, caused the issue to show up. Forcing an app to re-save its configuration seems to have worked for some.
In Trunas scale, port 53 is used by the Incus system for internal DNS resolution.
It’s also the standard port for DNS traffic, Services like Pihole can also use this port.
I believe yhere is a thread or a note somewhere that mentions if you are running Pihole or another DNS service on scale, you may need to configure it to use a different port if it conflicts with the Incus system’s default setting and I think the Incus port also can be changed if there is a conflict. See: